The genus Isatis includes 107 species in Europe and the Mediterranean area, 72 species in Asia.
Isatis tinctoria is a biennial herbaceous plant, 1m to 1.50m tall, with branched stem. It grows better in rich or calcareous soils and sunny lands.
The flowers are small, yellow and grow in clusters. Flowering occurs from May to June. The black fruits are oval.
The plant allows several harvests per year if the soil and sun exposure are good.
Its use as a dye plant is very old and widespread in the world. There are traces of its use since prehistoric times in Europe; its cultivation probably dates back to very ancient times. Egypt was a very ancient center of pastel dyeing.
Indigo plants represent about one thousand species in the world. Isatis tinctoria is the indigo plant which was the most widely used.
The roots and leaves are also used for medicinal purposes for their healing properties. The seed oil is used in the industry. The plant was also used as forage or pasture. In the Middle East, mixed with henna, it is used to dye hair.
The leaves contain a colorless glycoside of indoxyline, isatan B. By acid or enzymatic hydrolysis (fermentation) this one releases indoxyl which oxidizes by contact with oxygen from the air into dihydro-indigo (greenish) and then into indigotine (blue).
The leaf also contains but in much smaller amount, an other glycoside capable of providing indoxyl, indican.
Blues are used as basis for greens and violets dyeing (purple imitation).